The Securities and Exchange Commission has not necessarily reviewed the information in this filing and has not determined if it is accurate and complete.
The reader should not assume that the information is accurate and complete.

UNITED STATES SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549
FORM 13F

FORM 13F INFORMATION TABLE
OMB APPROVAL
OMB Number: 3235-0006
Estimated average burden
hours per response: 23.8
COLUMN 1 COLUMN 2 COLUMN 3 COLUMN 4 COLUMN 5 COLUMN 6 COLUMN 7 COLUMN 8
VALUE SHRS OR SH/ PUT/ INVESTMENT OTHER VOTING AUTHORITY
NAME OF ISSUER TITLE OF CLASS CUSIP FIGI (to the nearest dollar) PRN AMT PRN CALL DISCRETION MANAGER SOLE SHARED NONE
ABBOTT LABORATORIES COMMON STOCK 002824100   456,990 4,513 SH   DFND 1 4,513 0 0
ABBVIE INC COMMON STOCK 00287Y109   486,081 3,050 SH   DFND 1 3,050 0 0
ABBVIE INC COMMON STOCK 00287Y109   15,937 100 SH   OTR 1 100 0 0
ACTIVISION BLIZZARD INC COMMON STOCK 00507V109   309,408 3,615 SH   DFND 1 3,615 0 0
ACTIVISION BLIZZARD INC COMMON STOCK 00507V109   11,041 129 SH   OTR 1 129 0 0
ALPHABET INC COMMON STOCK 02079K305   581,407 5,605 SH   DFND 1 5,605 0 0
ALPHABET INC COMMON STOCK 02079K305   14,522 140 SH   OTR 1 140 0 0
AMAZON COM INC COMMON STOCK 023135106   890,670 8,623 SH   DFND 1 8,623 0 0
AMAZON COM INC COMMON STOCK 023135106   8,263 80 SH   OTR 1 80 0 0
APPLE INC COMMON STOCK 037833100   4,882,038 29,606 SH   DFND 1 29,606 0 0
APPLE INC COMMON STOCK 037833100   207,280 1,257 SH   OTR 1 1,257 0 0
APPLIED MATERIALS DELAWARE COMMON STOCK 038222105   374,020 3,045 SH   DFND 1 3,045 0 0
APPLIED MATERIALS DELAWARE COMMON STOCK 038222105   47,290 385 SH   OTR 1 385 0 0
BP AMOCO PLC SPONS ADR COMMON STOCK 055622104   287,092 7,567 SH   DFND 1 7,567 0 0
BANK OF AMERICA COMMON STOCK 060505104   760,017 26,574 SH   DFND 1 26,574 0 0
BIOLIFE SOLUTIONS INC COMMON STOCK 09062W204   1,060,856 48,775 SH   DFND 1 8,494 0 40,281
BRISTOL MYERS SQUIBB COMMON STOCK 110122108   422,377 6,094 SH   DFND 1 6,094 0 0
BRISTOL MYERS SQUIBB COMMON STOCK 110122108   46,438 670 SH   OTR 1 670 0 0
BROADCOM INC COMMON STOCK 11135F101   486,931 759 SH   DFND 1 759 0 0
CSX CORP COMMON STOCK 126408103   657,097 21,947 SH   DFND 1 21,947 0 0
CSX CORP COMMON STOCK 126408103   47,006 1,570 SH   OTR 1 1,570 0 0
CVS HEALTH CORPORATION COMMON STOCK 126650100   372,146 5,008 SH   DFND 1 5,008 0 0
CHARTER COMMUNICATIONS INC NEW COMMON STOCK 16119P108   256,764 718 SH   DFND 1 718 0 0
CHEVRON CORPORATION COMMON STOCK 166764100   4,300,246 26,356 SH   DFND 1 26,356 0 0
CHEVRON CORPORATION COMMON STOCK 166764100   24,474 150 SH   OTR 1 150 0 0
CISCO SYSTEMS COMMON STOCK 17275R102   351,653 6,727 SH   DFND 1 6,727 0 0
CISCO SYSTEMS COMMON STOCK 17275R102   23,524 450 SH   OTR 1 450 0 0
CINTAS CORPORATION COMMON STOCK 172908105   296,116 640 SH   DFND 1 640 0 0
CITIGROUP INC COMMON STOCK 172967424   200,924 4,285 SH   DFND 1 4,285 0 0
CITIGROUP INC COMMON STOCK 172967424   9,378 200 SH   OTR 1 200 0 0
CITIZENS FINANCIAL GROUP INC COMMON STOCK 174610105   204,117 6,721 SH   DFND 1 6,721 0 0
CLOROX COMMON STOCK 189054109   205,712 1,300 SH   DFND 1 1,300 0 0
CLOROX COMMON STOCK 189054109   15,824 100 SH   OTR 1 100 0 0
COCA COLA COMMON STOCK 191216100   359,340 5,793 SH   DFND 1 5,793 0 0
COMCAST CORP NEW COMMON STOCK 20030N101   203,159 5,359 SH   DFND 1 5,359 0 0
CONOCOPHILLIPS COMMON STOCK 20825C104   269,356 2,715 SH   DFND 1 2,715 0 0
CONOCOPHILLIPS COMMON STOCK 20825C104   16,866 170 SH   OTR 1 170 0 0
CORNING INC COMMON STOCK 219350105   224,487 6,363 SH   DFND 1 6,193 0 170
CORNING INC COMMON STOCK 219350105   10,584 300 SH   OTR 1 300 0 0
COSTCO WHOLESALE CORP COMMON STOCK 22160K105   705,557 1,420 SH   DFND 1 1,420 0 0
D R HORTON INC COMMON STOCK 23331A109   392,714 4,020 SH   DFND 1 4,020 0 0
DANAHER CORP COMMON STOCK 235851102   468,292 1,858 SH   DFND 1 1,858 0 0
DANAHER CORP COMMON STOCK 235851102   12,854 51 SH   OTR 1 51 0 0
DEERE & CO COMMON STOCK 244199105   1,347,229 3,263 SH   DFND 1 3,263 0 0
EDISON INTERNATIONAL COMMON STOCK 281020107   200,405 2,839 SH   DFND 1 2,839 0 0
ESCALADE INC COMMON STOCK 296056104   1,031,227 70,632 SH   DFND 1 70,632 0 0
EXXON MOBIL CORPORATION COMMON STOCK 30231G102   1,876,172 17,109 SH   DFND 1 17,109 0 0
EXXON MOBIL CORPORATION COMMON STOCK 30231G102   93,211 850 SH   OTR 1 850 0 0
META PLATFORMS INC COMMON STOCK 30303M102   748,788 3,533 SH   DFND 1 3,533 0 0
FISERV COMMON STOCK 337738108   914,416 8,090 SH   DFND 1 8,090 0 0
GENERAL DYNAMICS COMMON STOCK 369550108   346,193 1,517 SH   DFND 1 1,517 0 0
GENUINE PARTS CO COMMON STOCK 372460105   725,622 4,337 SH   DFND 1 4,337 0 0
HOME DEPOT INC COMMON STOCK 437076102   1,028,787 3,486 SH   DFND 1 3,486 0 0
HOME DEPOT INC COMMON STOCK 437076102   36,890 125 SH   OTR 1 125 0 0
HONEYWELL INTERNATIONAL INC COMMON STOCK 438516106   210,232 1,100 SH   DFND 1 1,100 0 0
ILLINOIS TOOL WORKS COMMON STOCK 452308109   235,904 969 SH   DFND 1 969 0 0
IBM COMMON STOCK 459200101   335,854 2,562 SH   DFND 1 2,562 0 0
IBM COMMON STOCK 459200101   52,436 400 SH   OTR 1 400 0 0
INVESCO MUTUAL FUNDS - 46090E103   392,496 1,223 SH   DFND 1 1,223 0 0
INVESCO MUTUAL FUNDS - 46137V357   291,698 2,017 SH   DFND 1 2,017 0 0
INVESCO MUTUAL FUNDS - 46138J619   785,727 16,901 SH   DFND 1 16,901 0 0
ISHARES MUTUAL FUNDS - 464287101   318,903 1,705 SH   DFND 1 1,705 0 0
ISHARES MUTUAL FUNDS - 464287168   561,879 4,795 SH   DFND 1 4,795 0 0
ISHARES MUTUAL FUNDS - 464287200   12,400,230 30,165 SH   DFND 1 30,165 0 0
ISHARES MUTUAL FUNDS - 464287200   188,275 458 SH   OTR 1 458 0 0
ISHARES MUTUAL FUNDS - 464287234   583,456 14,786 SH   DFND 1 14,786 0 0
ISHARES MUTUAL FUNDS - 464287309   6,957,691 108,901 SH   DFND 1 108,901 0 0
ISHARES MUTUAL FUNDS - 464287309   75,326 1,179 SH   OTR 1 1,179 0 0
ISHARES MUTUAL FUNDS - 464287408   1,817,630 11,977 SH   DFND 1 11,977 0 0
ISHARES MUTUAL FUNDS - 464287457   2,966,635 36,108 SH   DFND 1 36,108 0 0
ISHARES MUTUAL FUNDS - 464287457   6,326 77 SH   OTR 1 77 0 0
ISHARES MUTUAL FUNDS - 464287465   1,451,641 20,297 SH   DFND 1 20,297 0 0
ISHARES MUTUAL FUNDS - 464287499   1,863,926 26,658 SH   DFND 1 26,658 0 0
ISHARES MUTUAL FUNDS - 464287499   59,572 852 SH   OTR 1 852 0 0
ISHARES MUTUAL FUNDS - 464287507   9,541,346 38,141 SH   DFND 1 37,826 0 315
ISHARES MUTUAL FUNDS - 464287549   204,950 604 SH   DFND 1 604 0 0
ISHARES MUTUAL FUNDS - 464287614   229,182 938 SH   DFND 1 938 0 0
ISHARES MUTUAL FUNDS - 464287622   3,219,888 14,296 SH   DFND 1 14,296 0 0
ISHARES MUTUAL FUNDS - 464287804   5,396,354 55,805 SH   DFND 1 55,205 0 600
ISHARES MUTUAL FUNDS - 464287804   15,665 162 SH   OTR 1 162 0 0
ISHARES MUTUAL FUNDS - 464288448   401,299 14,598 SH   DFND 1 14,598 0 0
ISHARES MUTUAL FUNDS - 464288513   474,227 6,277 SH   OTR 1 6,277 0 0
ISHARES MUTUAL FUNDS - 464288570   514,577 6,598 SH   DFND 1 5,341 0 1,257
ISHARES MUTUAL FUNDS - 464288588   370,205 3,908 SH   DFND 1 3,908 0 0
ISHARES MUTUAL FUNDS - 464288638   3,654,622 71,268 SH   DFND 1 71,268 0 0
ISHARES MUTUAL FUNDS - 464288638   18,512 361 SH   OTR 1 361 0 0
ISHARES MUTUAL FUNDS - 464288646   1,946,298 38,510 SH   DFND 1 38,510 0 0
ISHARES MUTUAL FUNDS - 464288646   19,306 382 SH   OTR 1 382 0 0
ISHARES MUTUAL FUNDS - 464288661   821,551 6,983 SH   DFND 1 6,983 0 0
ISHARES MUTUAL FUNDS - 464288661   495,189 4,209 SH   OTR 1 4,209 0 0
ISHARES MUTUAL FUNDS - 464288802   506,257 5,768 SH   DFND 1 4,320 0 1,448
ISHARES MUTUAL FUNDS - 46432F842   1,297,157 19,404 SH   DFND 1 19,404 0 0
ISHARES MUTUAL FUNDS - 46432F842   45,257 677 SH   OTR 1 677 0 0
ISHARES MUTUAL FUNDS - 46432F859   4,301,489 91,220 SH   DFND 1 91,220 0 0
ISHARES MUTUAL FUNDS - 46432F859   38,431 815 SH   OTR 1 815 0 0
JP MORGAN CHASE & CO COMMON STOCK 46625H100   1,537,923 11,802 SH   DFND 1 11,613 0 189
JP MORGAN CHASE & CO COMMON STOCK 46625H100   26,062 200 SH   OTR 1 200 0 0
JP MORGAN MUTUAL FUNDS - 46641Q332   321,157 5,882 SH   DFND 1 5,882 0 0
JOHNSON & JOHNSON COMMON STOCK 478160104   1,187,765 7,663 SH   DFND 1 7,663 0 0
JOHNSON & JOHNSON COMMON STOCK 478160104   60,450 390 SH   OTR 1 390 0 0
KROGER CO COMMON STOCK 501044101   371,016 7,515 SH   DFND 1 7,515 0 0
LENNOX INTL COMMON STOCK 526107107   568,898 2,264 SH   DFND 1 2,264 0 0
ELI LILLY & CO COMMON STOCK 532457108   868,509 2,529 SH   DFND 1 2,529 0 0
LOCKHEED MARTIN CORP COMMON STOCK 539830109   210,365 445 SH   DFND 1 445 0 0
LOWES COMPANY COMMON STOCK 548661107   733,892 3,670 SH   DFND 1 3,670 0 0
MCDONALDS COMMON STOCK 580135101   881,054 3,151 SH   DFND 1 3,151 0 0
MCDONALDS COMMON STOCK 580135101   20,971 75 SH   OTR 1 75 0 0
MERCK & CO COMMON STOCK 58933Y105   1,040,922 9,784 SH   DFND 1 9,784 0 0
MERCK & CO COMMON STOCK 58933Y105   92,027 865 SH   OTR 1 865 0 0
METLIFE COMMON STOCK 59156R108   258,064 4,454 SH   DFND 1 4,454 0 0
METLIFE COMMON STOCK 59156R108   8,691 150 SH   OTR 1 150 0 0
MICROSOFT CORP COMMON STOCK 594918104   2,379,637 8,254 SH   DFND 1 8,254 0 0
MICROSOFT CORP COMMON STOCK 594918104   18,740 65 SH   OTR 1 65 0 0
MORGAN STANLEY COMMON STOCK 617446448   415,733 4,735 SH   DFND 1 4,735 0 0
NEXTERA ENERGY INC COMMON STOCK 65339F101   440,512 5,715 SH   DFND 1 5,715 0 0
NIKE CLASS B COMMON STOCK 654106103   206,649 1,685 SH   DFND 1 1,685 0 0
NVIDIA CORPORATION COMMON STOCK 67066G104   452,210 1,628 SH   DFND 1 1,628 0 0
NVIDIA CORPORATION COMMON STOCK 67066G104   23,333 84 SH   OTR 1 84 0 0
OMNICOM GROUP INC. COMMON STOCK 681919106   241,510 2,560 SH   DFND 1 2,560 0 0
ONEOK INC COMMON STOCK 682680103   252,572 3,975 SH   DFND 1 3,975 0 0
ORACLE CORPORATION COMMON STOCK 68389X105   888,966 9,567 SH   DFND 1 9,567 0 0
PPG INDUSTRIES INC COMMON STOCK 693506107   290,537 2,175 SH   DFND 1 2,175 0 0
PAYCHEX INC COMMON STOCK 704326107   216,118 1,886 SH   DFND 1 1,886 0 0
PEOPLES BANCORP INC COMMON STOCK 709789101   15,662,679 608,259 SH   DFND 1 16,512 591,747 0
PEOPLES BANCORP INC COMMON STOCK 709789101   2,575 100 SH   OTR 1 0 100 0
PEPSICO INC COMMON STOCK 713448108   809,053 4,438 SH   DFND 1 4,438 0 0
PEPSICO INC COMMON STOCK 713448108   35,002 192 SH   OTR 1 192 0 0
PFIZER INC COMMON STOCK 717081103   267,159 6,548 SH   DFND 1 6,548 0 0
PFIZER INC COMMON STOCK 717081103   68,340 1,675 SH   OTR 1 1,675 0 0
PHILIP MORRIS INTERNATIONAL INC COMMON STOCK 718172109   211,715 2,177 SH   DFND 1 2,177 0 0
PHILIP MORRIS INTERNATIONAL INC COMMON STOCK 718172109   19,450 200 SH   OTR 1 200 0 0
PHILLIPS 66 COMMON STOCK 718546104   781,644 7,710 SH   DFND 1 7,710 0 0
PHILLIPS 66 COMMON STOCK 718546104   10,138 100 SH   OTR 1 100 0 0
PIONEER NATURAL RESOURCES COMMON STOCK 723787107   236,919 1,160 SH   DFND 1 1,160 0 0
PROCTER & GAMBLE CO COMMON STOCK 742718109   2,979,303 20,037 SH   DFND 1 20,037 0 0
PROCTER & GAMBLE CO COMMON STOCK 742718109   144,824 974 SH   OTR 1 974 0 0
PROGRESSIVE CORP COMMON STOCK 743315103   229,038 1,601 SH   DFND 1 1,601 0 0
PRUDENTIAL FINANCIAL INC COMMON STOCK 744320102   316,813 3,829 SH   DFND 1 3,829 0 0
PRUDENTIAL FINANCIAL INC COMMON STOCK 744320102   8,274 100 SH   OTR 1 100 0 0
RAYTHEON TECHNOLOGIES CORP COMMON STOCK 75513E101   237,775 2,428 SH   DFND 1 2,428 0 0
RAYTHEON TECHNOLOGIES CORP COMMON STOCK 75513E101   9,793 100 SH   OTR 1 100 0 0
RIO TINTO PLC ADR COMMON STOCK 767204100   214,719 3,130 SH   DFND 1 3,130 0 0
SPDR MUTUAL FUNDS - 78462F103   6,043,007 14,761 SH   DFND 1 14,761 0 0
SPDR MUTUAL FUNDS - 78464A474   245,544 8,287 SH   DFND 1 8,287 0 0
SPDR MUTUAL FUNDS - 78464A763   741,641 5,995 SH   DFND 1 5,995 0 0
SPDR MUTUAL FUNDS - 78464A763   108,989 881 SH   OTR 1 881 0 0
SPDR MUTUAL FUNDS - 78468R408   248,350 9,998 SH   DFND 1 9,998 0 0
SPDR MUTUAL FUNDS - 78468R408   225,895 9,094 SH   OTR 1 9,094 0 0
SCHWAB MUTUAL FUNDS - 808524797   4,134,199 56,509 SH   DFND 1 56,509 0 0
SHERWIN WILLIAMS CO. COMMON COMMON STOCK 824348106   229,266 1,020 SH   DFND 1 1,020 0 0
STRYKER COMMON STOCK 863667101   378,534 1,326 SH   DFND 1 1,326 0 0
TEXAS INSTRUMENTS INC COMMON STOCK 882508104   343,372 1,846 SH   DFND 1 1,846 0 0
TEXAS INSTRUMENTS INC COMMON STOCK 882508104   23,251 125 SH   OTR 1 125 0 0
TRUIST FINANCIAL CORPORATION COMMON STOCK 89832Q109   314,506 9,223 SH   DFND 1 9,223 0 0
TRUIST FINANCIAL CORPORATION COMMON STOCK 89832Q109   6,615 194 SH   OTR 1 194 0 0
UNION PACIFIC CORP COMMON STOCK 907818108   725,140 3,603 SH   DFND 1 3,603 0 0
UNION PACIFIC CORP COMMON STOCK 907818108   20,126 100 SH   OTR 1 100 0 0
UNITED BANKSHARES INC COMMON STOCK 909907107   247,950 7,044 SH   DFND 1 7,044 0 0
UNITEDHEALTH GROUP INC COMMON STOCK 91324P102   547,262 1,158 SH   DFND 1 1,158 0 0
UNITEDHEALTH GROUP INC COMMON STOCK 91324P102   23,630 50 SH   OTR 1 50 0 0
VANGUARD MUTUAL FUNDS - 921943858   3,153,364 69,811 SH   DFND 1 69,811 0 0
VANGUARD MUTUAL FUNDS - 922042858   346,468 8,576 SH   DFND 1 8,576 0 0
VANGUARD MUTUAL FUNDS - 922042858   10,302 255 SH   OTR 1 255 0 0
VANGUARD MUTUAL FUNDS - 92204A702   441,748 1,146 SH   DFND 1 1,146 0 0
VANGUARD MUTUAL FUNDS - 92206C706   5,877,395 98,022 SH   DFND 1 98,022 0 0
VANGUARD MUTUAL FUNDS - 922908769   236,960 1,161 SH   DFND 1 1,161 0 0
VERIZON COMMUNICATIONS COMMON STOCK 92343V104   273,436 7,031 SH   DFND 1 7,031 0 0
VERIZON COMMUNICATIONS COMMON STOCK 92343V104   19,445 500 SH   OTR 1 500 0 0
VISA INC COMMON STOCK 92826C839   293,101 1,300 SH   DFND 1 1,300 0 0
VISA INC COMMON STOCK 92826C839   11,724 52 SH   OTR 1 52 0 0
LINDE PLC FOREIGN STOCK G54950103   270,490 761 SH   DFND 1 761 0 0
MEDTRONIC PLC COMMON STOCK G5960L103   321,351 3,986 SH   DFND 1 3,986 0 0